3-Phenylacetylamino-2, 6-Piperidinedione Inhibition of Rat Nb2 Lymphoma Cell Mitogenesis

Joseph C. Wood, John A. Copland, Thomas G. Muldoon, Lawrence B. Hendry

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

3-Phenylacetylamino-2,6-piperidinedione (A10), an amino acid analog, has been reported to possess antineoplastic activity against certain neoplastic tissues. The antimitogenic properties of A10 were studied by determining its effect on prolactin (PRL)- and interleukin 2 (IL-2)-stimulated mitogenic responses in the rat Nb2 lymphoma cell line. The addition of A10 (1-12 mM) to PRL (0.4 ng/ml)-stimulated cells inhibited growth in a dose-dependent manner. DNA synthesis patterns studied by thymidine incorporation demonstrated that A10 was significantly inhibitory (25% at 20 hr; 50% at 40 hr, P < 0.01). IL-2 stimulation of mitogenesis was also sensitive to A10 inhibition. The inhibition of PRL stimulated mitogenesis was reversible when A10 was removed after 24 hr of culture and A10 showed no toxicity in a chromium release assay. These data suggest that A10 effects may be cytostatic, rather than cytotoxic.

Original languageEnglish (US)
Pages (from-to)404-408
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume197
Issue number4
DOIs
StatePublished - Sep 1991

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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