17β-estradiol-dependent activation of signal transducer and activator of transcription-1 in human fetal osteoblasts is dependent on Src kinase activity

Angela M. Kennedy, Kristen L. Shogren, Minzhi Zhang, Russell T. Turner, Thomas C. Spelsberg, Avudaiappan Maran

Research output: Contribution to journalArticle

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Abstract

Estrogen is essential for normal growth and remodeling of bone. Although the mechanism of estrogen action on bone cells has been widely investigated, the full spectrum of signal transduction pathways activated by estrogen is unknown. In this report, we investigate the effects of the gonadal hormone 17β-estradiol on the regulation of signal transducer and activator of transcription-1 (Stat1) protein in cultured human fetal osteoblast cells, devoid of the classical estrogen receptors (ERs). 17β-Estradiol (10 nM) led to rapid (within 15 min) activation of Stat1 protein as indicated by increases in tyrosine phosphorylation and DNA binding activity. Also, 17β-estradiol increased γ-activated sequence-dependent transcription in transient transfection assays, suggesting an increase in Stat protein-dependent transcription. Estrogen-dependent Stat1 activation was blocked in cells that transiently express dominant-negative Stat1 mutant protein. Activation of Stat1 by 17β-estradiol was not inhibited by ER antagonist ICI 182,780, providing further evidence that it is not dependent on classical ERs. 17β-Estradiol induced rapid (within 15 min) Stat1 phosphorylation and stimulated γ-activated sequence-dependent transcription in ER-negative breast cancer cells, indicating that these results are not unique to bone cells. The rapid estrogenic effect involving the phosphorylation and activation of Stat1 was blocked in the presence of Src family kinase inhibitor PP2; activated Stat1 was associated with Src protein in estrogen-treated cells. These findings indicate the requirement for Src kinase pathways in estrogen-mediated Stat1 activation. Thus, the ER-independent activation of Stat1 in 17β-estradiol-treated osteoblast and breast cancer cells may partially mediate the actions of estrogen on target cells.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalEndocrinology
Volume146
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

STAT1 Transcription Factor
src-Family Kinases
Osteoblasts
Estradiol
Estrogens
Replication Protein C
Estrogen Receptors
STAT Transcription Factors
Phosphorylation
Transcriptional Activation
Breast Neoplasms
Gonadal Hormones
Bone and Bones
Bone Remodeling
Bone Development
Mutant Proteins
Transfection
Tyrosine
Signal Transduction
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

17β-estradiol-dependent activation of signal transducer and activator of transcription-1 in human fetal osteoblasts is dependent on Src kinase activity. / Kennedy, Angela M.; Shogren, Kristen L.; Zhang, Minzhi; Turner, Russell T.; Spelsberg, Thomas C.; Maran, Avudaiappan.

In: Endocrinology, Vol. 146, No. 1, 01.2005, p. 201-207.

Research output: Contribution to journalArticle

Kennedy, Angela M. ; Shogren, Kristen L. ; Zhang, Minzhi ; Turner, Russell T. ; Spelsberg, Thomas C. ; Maran, Avudaiappan. / 17β-estradiol-dependent activation of signal transducer and activator of transcription-1 in human fetal osteoblasts is dependent on Src kinase activity. In: Endocrinology. 2005 ; Vol. 146, No. 1. pp. 201-207.
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