1,25-dihydroxyvitamin D3 receptors in the central nervous system of the rat embryo

Timothy D. Veenstra, Kirsten Prüfer, Carol Koenigsberger, William Stephen Brimijoin, Joseph Peter Grande, Rajiv Kumar

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

We have mapped areas within the central nervous system (CNS) of the developing fetal rat which immunostain for the 1,25-dihydroxyvitamin D3 receptor (VDR). The VDR was detected from days 12 to 21 of gestation throughout the CNS; immunostaining was particularly intense in the neuroepithelium and within the differentiating fields of various areas of the brain. Cells within the spinal cord, dorsal root, and other ganglia exhibited positive staining for the VDR. The intensity of staining for the VDR diminished or disappeared in the neuroepithelium throughout the CNS during the later days of development, while in the differentiating fields single VDR immunoreactive cells were observed. The presence of the VDR in the CNS was confirmed by in situ hybridization and RNA-based polymerase chain reaction methods with di-deoxy sequencing of the resultant DNA product. These results support the hypothesis that 1,25-dihydroxyvitamin D3, through interactions with the VDR, may play a role in the development of the CNS.

Original languageEnglish (US)
Pages (from-to)193-205
Number of pages13
JournalBrain Research
Volume804
Issue number2
DOIs
StatePublished - Sep 7 1998

Fingerprint

Calcitriol Receptors
Embryonic Structures
Central Nervous System
Staining and Labeling
Calcitriol
Spinal Ganglia
DNA-Directed RNA Polymerases
DNA Sequence Analysis
In Situ Hybridization
Spinal Cord
Pregnancy
Polymerase Chain Reaction
Brain

Keywords

  • 1,25-Dihydroxyvitamin D receptor
  • Central nervous SYstem
  • Development
  • Fetal
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

1,25-dihydroxyvitamin D3 receptors in the central nervous system of the rat embryo. / Veenstra, Timothy D.; Prüfer, Kirsten; Koenigsberger, Carol; Brimijoin, William Stephen; Grande, Joseph Peter; Kumar, Rajiv.

In: Brain Research, Vol. 804, No. 2, 07.09.1998, p. 193-205.

Research output: Contribution to journalArticle

Veenstra, Timothy D. ; Prüfer, Kirsten ; Koenigsberger, Carol ; Brimijoin, William Stephen ; Grande, Joseph Peter ; Kumar, Rajiv. / 1,25-dihydroxyvitamin D3 receptors in the central nervous system of the rat embryo. In: Brain Research. 1998 ; Vol. 804, No. 2. pp. 193-205.
@article{8f9d0ef232c84485873820d559928533,
title = "1,25-dihydroxyvitamin D3 receptors in the central nervous system of the rat embryo",
abstract = "We have mapped areas within the central nervous system (CNS) of the developing fetal rat which immunostain for the 1,25-dihydroxyvitamin D3 receptor (VDR). The VDR was detected from days 12 to 21 of gestation throughout the CNS; immunostaining was particularly intense in the neuroepithelium and within the differentiating fields of various areas of the brain. Cells within the spinal cord, dorsal root, and other ganglia exhibited positive staining for the VDR. The intensity of staining for the VDR diminished or disappeared in the neuroepithelium throughout the CNS during the later days of development, while in the differentiating fields single VDR immunoreactive cells were observed. The presence of the VDR in the CNS was confirmed by in situ hybridization and RNA-based polymerase chain reaction methods with di-deoxy sequencing of the resultant DNA product. These results support the hypothesis that 1,25-dihydroxyvitamin D3, through interactions with the VDR, may play a role in the development of the CNS.",
keywords = "1,25-Dihydroxyvitamin D receptor, Central nervous SYstem, Development, Fetal, Rat",
author = "Veenstra, {Timothy D.} and Kirsten Pr{\"u}fer and Carol Koenigsberger and Brimijoin, {William Stephen} and Grande, {Joseph Peter} and Rajiv Kumar",
year = "1998",
month = "9",
day = "7",
doi = "10.1016/S0006-8993(98)00565-4",
language = "English (US)",
volume = "804",
pages = "193--205",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - 1,25-dihydroxyvitamin D3 receptors in the central nervous system of the rat embryo

AU - Veenstra, Timothy D.

AU - Prüfer, Kirsten

AU - Koenigsberger, Carol

AU - Brimijoin, William Stephen

AU - Grande, Joseph Peter

AU - Kumar, Rajiv

PY - 1998/9/7

Y1 - 1998/9/7

N2 - We have mapped areas within the central nervous system (CNS) of the developing fetal rat which immunostain for the 1,25-dihydroxyvitamin D3 receptor (VDR). The VDR was detected from days 12 to 21 of gestation throughout the CNS; immunostaining was particularly intense in the neuroepithelium and within the differentiating fields of various areas of the brain. Cells within the spinal cord, dorsal root, and other ganglia exhibited positive staining for the VDR. The intensity of staining for the VDR diminished or disappeared in the neuroepithelium throughout the CNS during the later days of development, while in the differentiating fields single VDR immunoreactive cells were observed. The presence of the VDR in the CNS was confirmed by in situ hybridization and RNA-based polymerase chain reaction methods with di-deoxy sequencing of the resultant DNA product. These results support the hypothesis that 1,25-dihydroxyvitamin D3, through interactions with the VDR, may play a role in the development of the CNS.

AB - We have mapped areas within the central nervous system (CNS) of the developing fetal rat which immunostain for the 1,25-dihydroxyvitamin D3 receptor (VDR). The VDR was detected from days 12 to 21 of gestation throughout the CNS; immunostaining was particularly intense in the neuroepithelium and within the differentiating fields of various areas of the brain. Cells within the spinal cord, dorsal root, and other ganglia exhibited positive staining for the VDR. The intensity of staining for the VDR diminished or disappeared in the neuroepithelium throughout the CNS during the later days of development, while in the differentiating fields single VDR immunoreactive cells were observed. The presence of the VDR in the CNS was confirmed by in situ hybridization and RNA-based polymerase chain reaction methods with di-deoxy sequencing of the resultant DNA product. These results support the hypothesis that 1,25-dihydroxyvitamin D3, through interactions with the VDR, may play a role in the development of the CNS.

KW - 1,25-Dihydroxyvitamin D receptor

KW - Central nervous SYstem

KW - Development

KW - Fetal

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0032494263&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032494263&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(98)00565-4

DO - 10.1016/S0006-8993(98)00565-4

M3 - Article

C2 - 9757035

AN - SCOPUS:0032494263

VL - 804

SP - 193

EP - 205

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -