1,2,3,4,6-Penta-O-galloyl-beta-D-glucose reduces renal crystallization and oxidative stress in a hyperoxaluric rat model

Hyo Jung Lee, Soo Jin Jeong, Hyo Jeong Lee, Eun Ok Lee, Hyunsu Bae, John C Lieske, Sung Hoon Kim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Adhesion of calcium oxalate (CaOx) crystals to kidney cells may be a key event in the pathogenesis of kidney stones associated with marked hyperoxaluria. Previously, we found that 1,2,3,4,6-penta-O-galloyl-Β-D-glucose (PGG), isolated from a traditional medicinal herb, reduced CaOx crystal adhesion to renal epithelial cells by acting on the cells as well as on the crystal surface. Here we used the ethylene glycol (EG)-mediated hyperoxaluric rat model and found evidence of oxidant stress as indicated by decreases in the activities of the renal antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase, with increased kidney cell apoptosis and serum malondialdehyde levels, all evident by 21 days of EG treatment. These effects of hyperoxaluria were reversed by concurrent PGG treatment along with decreased urinary oxalate levels and CaOx supersaturation. Renal epithelial cell expression of the crystal binding molecule hyaluronan increased diffusely within 7 days of EG initiation, suggesting it is not a result of but precedes crystal deposition. Renal cell osteopontin (OPN) was also upregulated in EG-treated animals, and PGG significantly attenuated overexpression of both OPN and hyaluronan. Thus, our findings demonstrate that PGG reduces renal crystallization and oxidative renal cell injury, and may be a candidate chemopreventive agent for nephrolithiasis.

Original languageEnglish (US)
Pages (from-to)538-545
Number of pages8
JournalKidney International
Volume79
Issue number5
DOIs
StatePublished - Mar 2011

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Crystallization
Oxidative Stress
Kidney
Rubiaceae
Ethylene Glycol
Calcium Oxalate
Hyperoxaluria
Osteopontin
Hyaluronic Acid
Glucose
Epithelial Cells
Nephrolithiasis
Kidney Calculi
Oxalates
beta-penta-O-galloyl-glucose
Medicinal Plants
Glutathione Peroxidase
Malondialdehyde
Oxidants
Catalase

Keywords

  • calcium oxalate
  • hyaluronan
  • nephrolithiasis
  • oxalate
  • oxidative stress

ASJC Scopus subject areas

  • Nephrology

Cite this

1,2,3,4,6-Penta-O-galloyl-beta-D-glucose reduces renal crystallization and oxidative stress in a hyperoxaluric rat model. / Lee, Hyo Jung; Jeong, Soo Jin; Lee, Hyo Jeong; Lee, Eun Ok; Bae, Hyunsu; Lieske, John C; Kim, Sung Hoon.

In: Kidney International, Vol. 79, No. 5, 03.2011, p. 538-545.

Research output: Contribution to journalArticle

Lee, Hyo Jung ; Jeong, Soo Jin ; Lee, Hyo Jeong ; Lee, Eun Ok ; Bae, Hyunsu ; Lieske, John C ; Kim, Sung Hoon. / 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose reduces renal crystallization and oxidative stress in a hyperoxaluric rat model. In: Kidney International. 2011 ; Vol. 79, No. 5. pp. 538-545.
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