1α,25-Dihydroxyvitamin D3 (1α,25(OH)2D3), alters the proliferation of keratinocytes in cell culture. To assess whether changes mediated by 1α,25(OH)2D3 in keratinocyte growth are due to the release of transforming growth factors β (TGFs β), we measured TGF β1 and 2 in human keratinocytes treated with 1α,25(OH)2D3. We showed that the inhibition of keratinocyte growth by 1A,25(OH)2D3 was associated with a time- and dose-dependent increase in the concentrations of TGF β2 but not TGF β1. There was a strong correlation between the inhibition of cell growth and the increase in TGF β2 concentrations. Antibodies directed against TGF B1, 2 and 3 partially blocked the inhibition of cellular growth mediated by 1α,25(OH)2D3. We conclude that 1A,25(OH)2D3 inhibits human keratinocyte cell growth by a mechanism that involves, at least in part, an increase in the release of TGF β2.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Dec 13 1996|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology