1α,25-dihydvoxyvitamin D3 inhibits normal human keratinocyte growth by increasing transforming growth factor β2 release

James D. Haugen, Mark R. Pittelkow, Alan R. Zinsmeister, Rajiv Kumar

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

1α,25-Dihydroxyvitamin D3 (1α,25(OH)2D3), alters the proliferation of keratinocytes in cell culture. To assess whether changes mediated by 1α,25(OH)2D3 in keratinocyte growth are due to the release of transforming growth factors β (TGFs β), we measured TGF β1 and 2 in human keratinocytes treated with 1α,25(OH)2D3. We showed that the inhibition of keratinocyte growth by 1A,25(OH)2D3 was associated with a time- and dose-dependent increase in the concentrations of TGF β2 but not TGF β1. There was a strong correlation between the inhibition of cell growth and the increase in TGF β2 concentrations. Antibodies directed against TGF B1, 2 and 3 partially blocked the inhibition of cellular growth mediated by 1α,25(OH)2D3. We conclude that 1A,25(OH)2D3 inhibits human keratinocyte cell growth by a mechanism that involves, at least in part, an increase in the release of TGF β2.

Original languageEnglish (US)
Pages (from-to)618-623
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume229
Issue number2
DOIs
StatePublished - Dec 13 1996

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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