TY - JOUR
T1 - 1α,25-dihydvoxyvitamin D3 inhibits normal human keratinocyte growth by increasing transforming growth factor β2 release
AU - Haugen, James D.
AU - Pittelkow, Mark R.
AU - Zinsmeister, Alan R.
AU - Kumar, Rajiv
N1 - Funding Information:
1Supported by NIH Grant DK25409. 2Address all inquiries to: Dr. Rajiv Kumar, Mayo Clinic, 200 First St., SW, 911A Guggenheim Bldg., Rochester, MN 55905. Fax: 507-266-4710; E-mail: rkumar@mayo.edu.
PY - 1996/12/13
Y1 - 1996/12/13
N2 - 1α,25-Dihydroxyvitamin D3 (1α,25(OH)2D3), alters the proliferation of keratinocytes in cell culture. To assess whether changes mediated by 1α,25(OH)2D3 in keratinocyte growth are due to the release of transforming growth factors β (TGFs β), we measured TGF β1 and 2 in human keratinocytes treated with 1α,25(OH)2D3. We showed that the inhibition of keratinocyte growth by 1A,25(OH)2D3 was associated with a time- and dose-dependent increase in the concentrations of TGF β2 but not TGF β1. There was a strong correlation between the inhibition of cell growth and the increase in TGF β2 concentrations. Antibodies directed against TGF B1, 2 and 3 partially blocked the inhibition of cellular growth mediated by 1α,25(OH)2D3. We conclude that 1A,25(OH)2D3 inhibits human keratinocyte cell growth by a mechanism that involves, at least in part, an increase in the release of TGF β2.
AB - 1α,25-Dihydroxyvitamin D3 (1α,25(OH)2D3), alters the proliferation of keratinocytes in cell culture. To assess whether changes mediated by 1α,25(OH)2D3 in keratinocyte growth are due to the release of transforming growth factors β (TGFs β), we measured TGF β1 and 2 in human keratinocytes treated with 1α,25(OH)2D3. We showed that the inhibition of keratinocyte growth by 1A,25(OH)2D3 was associated with a time- and dose-dependent increase in the concentrations of TGF β2 but not TGF β1. There was a strong correlation between the inhibition of cell growth and the increase in TGF β2 concentrations. Antibodies directed against TGF B1, 2 and 3 partially blocked the inhibition of cellular growth mediated by 1α,25(OH)2D3. We conclude that 1A,25(OH)2D3 inhibits human keratinocyte cell growth by a mechanism that involves, at least in part, an increase in the release of TGF β2.
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U2 - 10.1006/bbrc.1996.1853
DO - 10.1006/bbrc.1996.1853
M3 - Article
C2 - 8954947
AN - SCOPUS:0030582709
SN - 0006-291X
VL - 229
SP - 618
EP - 623
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -