γH2AX expression as a potential biomarker differentiating between low and high grade cervical squamous intraepithelial lesions (SIL) and high risk HPV related SIL

Konstantinos Leventakos, Sotirios Tsiodras, Theodore Kelesidis, Maria Kefala, Christine Kottaridi, Aris Spathis, Alina Roxani Gouloumi, Abraham Pouliakis, Asimakis Pappas, Vasileios Sioulas, Charalambos Chrelias, Petros Karakitsos, Ioannis Panayiotides

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: γH2AX is a protein biomarker for double-stranded DNA breakage; its expression was studied in cervical squamous intraepithelial lesions and carcinomas. Methods: Immunostaining for phospho-γH2AX was performed in sections from histologically confirmed cervical SIL and carcinomas, as well as from normal cervices used as controls. In total, 275 cases were included in the study: 112 low grade SIL (LGSIL), 99 high grade SIL (HGSIL), 24 squamous cell carcinoma (SCC), 12 adenocarcinoma and 28 cervical specimens with no essential lesions. Correlation of histological grading, high risk vs. low risk HPV virus presence, activated vs. non-activated status (by high risk HPV mRNA expression) and γH2AX expression in both basal and surface segments of the squamous epithelium was performed. Results: Gradual increase of both basal and surface γH2AX expression was noted up from normal cervices to LGSIL harboring a low risk HPV type, to LGSIL harboring a high risk virus at a non-activated state (p<0.05). Thereafter, both basal and surface γH2AX expression dropped in LGSIL harboring a high risk virus at an activated state and in HGSIL. Conclusions: γH2AX could serve as a potential biomarker discriminating between LGSIL and HGSIL, as well as between LGSIL harboring high risk HPV at an activated state.

Original languageEnglish (US)
Article numbere0170626
JournalPloS one
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Biomarkers
lesions (animal)
biomarkers
Viruses
Squamous Cell Carcinoma
cervix
Carcinoma in Situ
Cervix Uteri
viruses
carcinoma
squamous cell carcinoma
adenocarcinoma
Squamous Intraepithelial Lesions of the Cervix
Adenocarcinoma
epithelium
Epithelium
Messenger RNA
DNA
Proteins
proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

γH2AX expression as a potential biomarker differentiating between low and high grade cervical squamous intraepithelial lesions (SIL) and high risk HPV related SIL. / Leventakos, Konstantinos; Tsiodras, Sotirios; Kelesidis, Theodore; Kefala, Maria; Kottaridi, Christine; Spathis, Aris; Gouloumi, Alina Roxani; Pouliakis, Abraham; Pappas, Asimakis; Sioulas, Vasileios; Chrelias, Charalambos; Karakitsos, Petros; Panayiotides, Ioannis.

In: PloS one, Vol. 12, No. 1, e0170626, 01.01.2017.

Research output: Contribution to journalArticle

Leventakos, K, Tsiodras, S, Kelesidis, T, Kefala, M, Kottaridi, C, Spathis, A, Gouloumi, AR, Pouliakis, A, Pappas, A, Sioulas, V, Chrelias, C, Karakitsos, P & Panayiotides, I 2017, 'γH2AX expression as a potential biomarker differentiating between low and high grade cervical squamous intraepithelial lesions (SIL) and high risk HPV related SIL', PloS one, vol. 12, no. 1, e0170626. https://doi.org/10.1371/journal.pone.0170626
Leventakos, Konstantinos ; Tsiodras, Sotirios ; Kelesidis, Theodore ; Kefala, Maria ; Kottaridi, Christine ; Spathis, Aris ; Gouloumi, Alina Roxani ; Pouliakis, Abraham ; Pappas, Asimakis ; Sioulas, Vasileios ; Chrelias, Charalambos ; Karakitsos, Petros ; Panayiotides, Ioannis. / γH2AX expression as a potential biomarker differentiating between low and high grade cervical squamous intraepithelial lesions (SIL) and high risk HPV related SIL. In: PloS one. 2017 ; Vol. 12, No. 1.
@article{8eaa7f0b66d4481e9f06469f8f2392c9,
title = "γH2AX expression as a potential biomarker differentiating between low and high grade cervical squamous intraepithelial lesions (SIL) and high risk HPV related SIL",
abstract = "Background: γH2AX is a protein biomarker for double-stranded DNA breakage; its expression was studied in cervical squamous intraepithelial lesions and carcinomas. Methods: Immunostaining for phospho-γH2AX was performed in sections from histologically confirmed cervical SIL and carcinomas, as well as from normal cervices used as controls. In total, 275 cases were included in the study: 112 low grade SIL (LGSIL), 99 high grade SIL (HGSIL), 24 squamous cell carcinoma (SCC), 12 adenocarcinoma and 28 cervical specimens with no essential lesions. Correlation of histological grading, high risk vs. low risk HPV virus presence, activated vs. non-activated status (by high risk HPV mRNA expression) and γH2AX expression in both basal and surface segments of the squamous epithelium was performed. Results: Gradual increase of both basal and surface γH2AX expression was noted up from normal cervices to LGSIL harboring a low risk HPV type, to LGSIL harboring a high risk virus at a non-activated state (p<0.05). Thereafter, both basal and surface γH2AX expression dropped in LGSIL harboring a high risk virus at an activated state and in HGSIL. Conclusions: γH2AX could serve as a potential biomarker discriminating between LGSIL and HGSIL, as well as between LGSIL harboring high risk HPV at an activated state.",
author = "Konstantinos Leventakos and Sotirios Tsiodras and Theodore Kelesidis and Maria Kefala and Christine Kottaridi and Aris Spathis and Gouloumi, {Alina Roxani} and Abraham Pouliakis and Asimakis Pappas and Vasileios Sioulas and Charalambos Chrelias and Petros Karakitsos and Ioannis Panayiotides",
year = "2017",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0170626",
language = "English (US)",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

TY - JOUR

T1 - γH2AX expression as a potential biomarker differentiating between low and high grade cervical squamous intraepithelial lesions (SIL) and high risk HPV related SIL

AU - Leventakos, Konstantinos

AU - Tsiodras, Sotirios

AU - Kelesidis, Theodore

AU - Kefala, Maria

AU - Kottaridi, Christine

AU - Spathis, Aris

AU - Gouloumi, Alina Roxani

AU - Pouliakis, Abraham

AU - Pappas, Asimakis

AU - Sioulas, Vasileios

AU - Chrelias, Charalambos

AU - Karakitsos, Petros

AU - Panayiotides, Ioannis

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: γH2AX is a protein biomarker for double-stranded DNA breakage; its expression was studied in cervical squamous intraepithelial lesions and carcinomas. Methods: Immunostaining for phospho-γH2AX was performed in sections from histologically confirmed cervical SIL and carcinomas, as well as from normal cervices used as controls. In total, 275 cases were included in the study: 112 low grade SIL (LGSIL), 99 high grade SIL (HGSIL), 24 squamous cell carcinoma (SCC), 12 adenocarcinoma and 28 cervical specimens with no essential lesions. Correlation of histological grading, high risk vs. low risk HPV virus presence, activated vs. non-activated status (by high risk HPV mRNA expression) and γH2AX expression in both basal and surface segments of the squamous epithelium was performed. Results: Gradual increase of both basal and surface γH2AX expression was noted up from normal cervices to LGSIL harboring a low risk HPV type, to LGSIL harboring a high risk virus at a non-activated state (p<0.05). Thereafter, both basal and surface γH2AX expression dropped in LGSIL harboring a high risk virus at an activated state and in HGSIL. Conclusions: γH2AX could serve as a potential biomarker discriminating between LGSIL and HGSIL, as well as between LGSIL harboring high risk HPV at an activated state.

AB - Background: γH2AX is a protein biomarker for double-stranded DNA breakage; its expression was studied in cervical squamous intraepithelial lesions and carcinomas. Methods: Immunostaining for phospho-γH2AX was performed in sections from histologically confirmed cervical SIL and carcinomas, as well as from normal cervices used as controls. In total, 275 cases were included in the study: 112 low grade SIL (LGSIL), 99 high grade SIL (HGSIL), 24 squamous cell carcinoma (SCC), 12 adenocarcinoma and 28 cervical specimens with no essential lesions. Correlation of histological grading, high risk vs. low risk HPV virus presence, activated vs. non-activated status (by high risk HPV mRNA expression) and γH2AX expression in both basal and surface segments of the squamous epithelium was performed. Results: Gradual increase of both basal and surface γH2AX expression was noted up from normal cervices to LGSIL harboring a low risk HPV type, to LGSIL harboring a high risk virus at a non-activated state (p<0.05). Thereafter, both basal and surface γH2AX expression dropped in LGSIL harboring a high risk virus at an activated state and in HGSIL. Conclusions: γH2AX could serve as a potential biomarker discriminating between LGSIL and HGSIL, as well as between LGSIL harboring high risk HPV at an activated state.

UR - http://www.scopus.com/inward/record.url?scp=85010755874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85010755874&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0170626

DO - 10.1371/journal.pone.0170626

M3 - Article

VL - 12

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e0170626

ER -