β subunit heterogeneity in N-type Ca2+ channels

Victoria E S Scott, Michel De Waard, Hongyan Liu, Christina A. Gurnett, David P. Venzke, Vanda A Lennon, Kevin P. Campbell

Research output: Contribution to journalArticle

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Abstract

The β subunit of the voltage-dependent Ca2+ channel is a cytoplasmic protein that interacts directly with an α1 subunit, thereby modulating the biophysical properties of the channel. Herein, we demonstrate that the α1B subunit of the N-type Ca2+ channel associates with several different β subunits. Polyclonal antibodies specific for three different β subunits immunoprecipitated 125I-ω-conotoxin GVIA binding from solubilized rabbit brain membranes. Enrichment of the N-type Ca2+ channels with an α1B subunit-specific monoclonal antibody showed the association of β1b, β3, and β4 subunits. Protein sequencing of tryptic peptides of the 57-kDa component of the purified N-type Ca2+ channel confirmed the presence of the β3 and β4 subunits. Each of the β subunits bound to the α1B subunit interaction domain with similar high affinity. Thus, our data demonstrate important heterogeneity in the β subunit composition of the N-type Ca2+ channels, which may be responsible for some of the diverse kinetic properties recorded from neurons.

Original languageEnglish (US)
Pages (from-to)3207-3212
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number6
StatePublished - Feb 9 1996

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Conotoxins
Protein Sequence Analysis
Monoclonal Antibodies
Rabbits
Neurons
Peptides
Membranes
Antibodies
Brain
Proteins
Association reactions
Kinetics
Electric potential
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Scott, V. E. S., De Waard, M., Liu, H., Gurnett, C. A., Venzke, D. P., Lennon, V. A., & Campbell, K. P. (1996). β subunit heterogeneity in N-type Ca2+ channels. Journal of Biological Chemistry, 271(6), 3207-3212.

β subunit heterogeneity in N-type Ca2+ channels. / Scott, Victoria E S; De Waard, Michel; Liu, Hongyan; Gurnett, Christina A.; Venzke, David P.; Lennon, Vanda A; Campbell, Kevin P.

In: Journal of Biological Chemistry, Vol. 271, No. 6, 09.02.1996, p. 3207-3212.

Research output: Contribution to journalArticle

Scott, VES, De Waard, M, Liu, H, Gurnett, CA, Venzke, DP, Lennon, VA & Campbell, KP 1996, 'β subunit heterogeneity in N-type Ca2+ channels', Journal of Biological Chemistry, vol. 271, no. 6, pp. 3207-3212.
Scott VES, De Waard M, Liu H, Gurnett CA, Venzke DP, Lennon VA et al. β subunit heterogeneity in N-type Ca2+ channels. Journal of Biological Chemistry. 1996 Feb 9;271(6):3207-3212.
Scott, Victoria E S ; De Waard, Michel ; Liu, Hongyan ; Gurnett, Christina A. ; Venzke, David P. ; Lennon, Vanda A ; Campbell, Kevin P. / β subunit heterogeneity in N-type Ca2+ channels. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 6. pp. 3207-3212.
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