β subunit heterogeneity in N-type Ca2+ channels

Victoria E.S. Scott, Michel De Waard, Hongyan Liu, Christina A. Gurnett, David P. Venzke, Vanda A. Lennon, Kevin P. Campbell

Research output: Contribution to journalArticle

119 Scopus citations

Abstract

The β subunit of the voltage-dependent Ca2+ channel is a cytoplasmic protein that interacts directly with an α1 subunit, thereby modulating the biophysical properties of the channel. Herein, we demonstrate that the α1B subunit of the N-type Ca2+ channel associates with several different β subunits. Polyclonal antibodies specific for three different β subunits immunoprecipitated 125I-ω-conotoxin GVIA binding from solubilized rabbit brain membranes. Enrichment of the N-type Ca2+ channels with an α1B subunit-specific monoclonal antibody showed the association of β1b, β3, and β4 subunits. Protein sequencing of tryptic peptides of the 57-kDa component of the purified N-type Ca2+ channel confirmed the presence of the β3 and β4 subunits. Each of the β subunits bound to the α1B subunit interaction domain with similar high affinity. Thus, our data demonstrate important heterogeneity in the β subunit composition of the N-type Ca2+ channels, which may be responsible for some of the diverse kinetic properties recorded from neurons.

Original languageEnglish (US)
Pages (from-to)3207-3212
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number6
DOIs
StatePublished - Feb 9 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Scott, V. E. S., De Waard, M., Liu, H., Gurnett, C. A., Venzke, D. P., Lennon, V. A., & Campbell, K. P. (1996). β subunit heterogeneity in N-type Ca2+ channels. Journal of Biological Chemistry, 271(6), 3207-3212. https://doi.org/10.1074/jbc.271.6.3207