β-Methyl-15-p-iodophenylpentadecanoic acid metabolism and kinetics in the isolated rat heart

Timothy R. DeGrado, James E. Holden, Chin K. Ng, David M. Raffel, S. John Gatley

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The use of 15-p-iodophenyl-β-methyl-pentadecanoic acid (βMe-IPPA) as an indicator of long chain fatty acid (LCFA) utilization in nuclear medicine studies was evaluated in the isolated, perfused, working rat heart. Time courses of radioctivity (residue curves) were obtained following bolus injections of both βMe-IPPA and its straight chain counterpart 15-p-iodophenyl-pentadecanoic acid (IPPA). IPPA kinetics clearly indicated flow independent impairment of fatty acid oxidation caused by the carnitine palmitoyltransferase I inhibitor 2[5(4-chlorophenyl)pentyl]oxirane-2-carboxylate (POCA). In contrast, βMe-IPPA kinetics were insenstive to changes in fatty acid oxidation rate and net utilization of long chain fatty acid. Analysis of radiolabeled species in coronary effluent and heart homogenates showed the methylated fatty acid to be readily incorporated into complex lipids but a poor substrate for oxidation. POCA did not significatly alter metabolism of the tracer, suggesting that the tracer is poorly metabolized beyond βMe-IPPA-CoA in the oxidative pathway.

Original languageEnglish (US)
Pages (from-to)78-80
Number of pages3
JournalEuropean Journal of Nuclear Medicine
Volume15
Issue number2
DOIs
StatePublished - Feb 1 1989

Keywords

  • Myocardium
  • Perfusion
  • Radioiodinated fatty acid
  • β-oxidation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'β-Methyl-15-p-iodophenylpentadecanoic acid metabolism and kinetics in the isolated rat heart'. Together they form a unique fingerprint.

  • Cite this