β-glucan-activated human b lymphocytes participate in innate immune responses by releasing proinflammatory cytokines and stimulating neutrophil chemotaxis

Mohamed F. Ali, Christopher B. Driscoll, Paula R. Walters, Andrew Harold Limper, Eva M Carmona Porquera

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

B lymphocytes play an essential regulatory role in the adaptive immune response through Ab production during infection. A less known function of B lymphocytes is their ability to respond directly to infectious Ags through stimulation of pattern recognition receptors expressed on their surfaces. b-Glucans are carbohydrates present in the cell wall of many pathogenic fungi that can be detected in the peripheral blood of patients during infection. They have been shown to participate in the innate inflammatory response, as they can directly activate peripheral macrophages and dendritic cells. However, their effect as direct stimulators of B lymphocytes has not been yet fully elucidated. The aim of this study was to examine the molecular mechanisms and cytokine profiles generated following b-glucan stimulation of B lymphocytes, compared with the well-established TLR-9 agonist CpG oligodeoxynucleotide (CpG), and study the participation of b-glucan-stimulated B cells in the innate immune response. In this article, we demonstrate that b-glucan-activated B lymphocytes upregulate proinflammatory cytokines (TNF-α, IL-6, and IL-8). Of interest, b-glucan, unlike CpG, had no effect on B lymphocyte proliferation or IgM production. When compared with CpG (TLR9 agonist), b-glucan-activated cells secreted significantly higher levels of IL-8. Furthermore, IL-8 secretion was partially mediated by Dectin-1 and required SYK, MAPKs, and the transcription factors NF-κB and AP-1. Moreover, we observed that conditioned media from b-glucan-stimulated B lymphocytes elicited neutrophil chemotaxis. These studies suggest that b-glucan-activated B lymphocytes have an important and novel role in fungal innate immune responses.

Original languageEnglish (US)
Pages (from-to)5318-5326
Number of pages9
JournalJournal of Immunology
Volume195
Issue number11
DOIs
StatePublished - Dec 1 2015

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Glucans
Chemotaxis
Innate Immunity
Neutrophils
B-Lymphocytes
Lymphocytes
Cytokines
Interleukin-8
B-Cell Activating Factor
Pattern Recognition Receptors
Aptitude
Oligodeoxyribonucleotides
Transcription Factor AP-1
Adaptive Immunity
Conditioned Culture Medium
Infection
Dendritic Cells
Cell Wall
Immunoglobulin M
Interleukin-6

ASJC Scopus subject areas

  • Immunology

Cite this

β-glucan-activated human b lymphocytes participate in innate immune responses by releasing proinflammatory cytokines and stimulating neutrophil chemotaxis. / Ali, Mohamed F.; Driscoll, Christopher B.; Walters, Paula R.; Limper, Andrew Harold; Carmona Porquera, Eva M.

In: Journal of Immunology, Vol. 195, No. 11, 01.12.2015, p. 5318-5326.

Research output: Contribution to journalArticle

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