β-globin gene promoter generates 5′ truncated transcripts in the embryonic/fetal erythroid environment

Khashayarsha Khazaie, Fotini Gounari, Michael Antoniou, Ernie Deboer, Frank Grosveld

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We report here the localisation of sequences responsible for the faulty expression of human β-globin gene in Putko and K562 cells. Complete β-globin gene introduced into these cells produces transcripts with abnormal 5′ ends, while cotransfected mouse H2 gene is expressed correctly. Using hybrid constructs of these two genes we demonstrate that aberrant activity is conferred by sequences 5′ of the β-globin gene. Thus β-globin promoter attached to the H2 coding sequence produces H2 transcripts with truncated 5′ ends. By introducing a series of deletions in the )β-globin promoter we restrict these sequences to the -77 / +28 base pair region spanning the CAAT element to the translation initiation she. These results are consistent with the lack of recognition of the β-globin gene major cap site in Putko and K562 cells. We suggest that inactivity of the adult giobin gene in the embryonic/fetal environment is at least in part conferred by sequences within the β-giobin gene promoter.

Original languageEnglish (US)
Pages (from-to)7199-7212
Number of pages14
JournalNucleic Acids Research
Volume14
Issue number18
DOIs
StatePublished - Sep 11 1986
Externally publishedYes

Fingerprint

Globins
Promoter
Genes
Gene
K562 Cells
Cell
Base Pairing
Deletion
Mouse
Coding
Series

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

β-globin gene promoter generates 5′ truncated transcripts in the embryonic/fetal erythroid environment. / Khazaie, Khashayarsha; Gounari, Fotini; Antoniou, Michael; Deboer, Ernie; Grosveld, Frank.

In: Nucleic Acids Research, Vol. 14, No. 18, 11.09.1986, p. 7199-7212.

Research output: Contribution to journalArticle

Khazaie, Khashayarsha ; Gounari, Fotini ; Antoniou, Michael ; Deboer, Ernie ; Grosveld, Frank. / β-globin gene promoter generates 5′ truncated transcripts in the embryonic/fetal erythroid environment. In: Nucleic Acids Research. 1986 ; Vol. 14, No. 18. pp. 7199-7212.
@article{8bfaa399684d4635ac3e62150acb9c18,
title = "β-globin gene promoter generates 5′ truncated transcripts in the embryonic/fetal erythroid environment",
abstract = "We report here the localisation of sequences responsible for the faulty expression of human β-globin gene in Putko and K562 cells. Complete β-globin gene introduced into these cells produces transcripts with abnormal 5′ ends, while cotransfected mouse H2 gene is expressed correctly. Using hybrid constructs of these two genes we demonstrate that aberrant activity is conferred by sequences 5′ of the β-globin gene. Thus β-globin promoter attached to the H2 coding sequence produces H2 transcripts with truncated 5′ ends. By introducing a series of deletions in the )β-globin promoter we restrict these sequences to the -77 / +28 base pair region spanning the CAAT element to the translation initiation she. These results are consistent with the lack of recognition of the β-globin gene major cap site in Putko and K562 cells. We suggest that inactivity of the adult giobin gene in the embryonic/fetal environment is at least in part conferred by sequences within the β-giobin gene promoter.",
author = "Khashayarsha Khazaie and Fotini Gounari and Michael Antoniou and Ernie Deboer and Frank Grosveld",
year = "1986",
month = "9",
day = "11",
doi = "10.1093/nar/14.18.7199",
language = "English (US)",
volume = "14",
pages = "7199--7212",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "18",

}

TY - JOUR

T1 - β-globin gene promoter generates 5′ truncated transcripts in the embryonic/fetal erythroid environment

AU - Khazaie, Khashayarsha

AU - Gounari, Fotini

AU - Antoniou, Michael

AU - Deboer, Ernie

AU - Grosveld, Frank

PY - 1986/9/11

Y1 - 1986/9/11

N2 - We report here the localisation of sequences responsible for the faulty expression of human β-globin gene in Putko and K562 cells. Complete β-globin gene introduced into these cells produces transcripts with abnormal 5′ ends, while cotransfected mouse H2 gene is expressed correctly. Using hybrid constructs of these two genes we demonstrate that aberrant activity is conferred by sequences 5′ of the β-globin gene. Thus β-globin promoter attached to the H2 coding sequence produces H2 transcripts with truncated 5′ ends. By introducing a series of deletions in the )β-globin promoter we restrict these sequences to the -77 / +28 base pair region spanning the CAAT element to the translation initiation she. These results are consistent with the lack of recognition of the β-globin gene major cap site in Putko and K562 cells. We suggest that inactivity of the adult giobin gene in the embryonic/fetal environment is at least in part conferred by sequences within the β-giobin gene promoter.

AB - We report here the localisation of sequences responsible for the faulty expression of human β-globin gene in Putko and K562 cells. Complete β-globin gene introduced into these cells produces transcripts with abnormal 5′ ends, while cotransfected mouse H2 gene is expressed correctly. Using hybrid constructs of these two genes we demonstrate that aberrant activity is conferred by sequences 5′ of the β-globin gene. Thus β-globin promoter attached to the H2 coding sequence produces H2 transcripts with truncated 5′ ends. By introducing a series of deletions in the )β-globin promoter we restrict these sequences to the -77 / +28 base pair region spanning the CAAT element to the translation initiation she. These results are consistent with the lack of recognition of the β-globin gene major cap site in Putko and K562 cells. We suggest that inactivity of the adult giobin gene in the embryonic/fetal environment is at least in part conferred by sequences within the β-giobin gene promoter.

UR - http://www.scopus.com/inward/record.url?scp=0023056962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023056962&partnerID=8YFLogxK

U2 - 10.1093/nar/14.18.7199

DO - 10.1093/nar/14.18.7199

M3 - Article

C2 - 2429259

AN - SCOPUS:0023056962

VL - 14

SP - 7199

EP - 7212

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 18

ER -