TY - JOUR
T1 - β-2-adrenergic stimulation of ornithine decarboxylase activity in porcine granulosa cells in vitro
AU - Veldhuis, Johannes D.
AU - Harrison, Timothy S.
AU - Hammond, James M.
N1 - Funding Information:
This work was supportedb y NIH Grant No. HD-10122 to J.M.H., HEW Grant No. HL-18995 to T.S.H., and a NIH ResearchS erviceA ward No. 1F 32 HD05755 from the National Institute of Child Health and Human Development, BethesdaM, D to J.D.V. We thank John F. Seatona nd Jacob D. Peuler for the catecholamindee terminationas,n d the ShamokinP ackingC orporation
PY - 1980/1/17
Y1 - 1980/1/17
N2 - Epinephrine, norepinephrine, and isoproterenol produced dose-dependent stimulation of ornithine decarboxylase (EC 4.1.1.7) activity in isolated porcine granulosa cells maintained under defined conditions in vitro. β- but not α-receptor-blocking agents prevented enzyme stimulation by catecholamines. Application of preferential β-1 and β-2-receptor antagonists and agonists localized the epinephrine effect to β-2-adrenergic mediation. Epinephrine action was enhanced by the phosphodiesterase inhibitor, 1-methyl-3-isobutyl-xanthine, but not by saturating concentrations of the cyclic AMP analogue, 8-bromocyclic AMP, of follicle-stimulating hormone, or of prostaglandin E2. However, stimulation by epinephrine was additive to that of luteinizing hormone. Follicular fluid obtained from immature Graafian follicles contined concentrations of norepinephrine and epinephrine active in vitro. Thus, catecholamines may participate in the regulation of ornithine decarboxylase activity in the ovary. Catecholamine effects may be mediated by β-2-receptors linked to the adenylate cyclase system.
AB - Epinephrine, norepinephrine, and isoproterenol produced dose-dependent stimulation of ornithine decarboxylase (EC 4.1.1.7) activity in isolated porcine granulosa cells maintained under defined conditions in vitro. β- but not α-receptor-blocking agents prevented enzyme stimulation by catecholamines. Application of preferential β-1 and β-2-receptor antagonists and agonists localized the epinephrine effect to β-2-adrenergic mediation. Epinephrine action was enhanced by the phosphodiesterase inhibitor, 1-methyl-3-isobutyl-xanthine, but not by saturating concentrations of the cyclic AMP analogue, 8-bromocyclic AMP, of follicle-stimulating hormone, or of prostaglandin E2. However, stimulation by epinephrine was additive to that of luteinizing hormone. Follicular fluid obtained from immature Graafian follicles contined concentrations of norepinephrine and epinephrine active in vitro. Thus, catecholamines may participate in the regulation of ornithine decarboxylase activity in the ovary. Catecholamine effects may be mediated by β-2-receptors linked to the adenylate cyclase system.
KW - (Porcine granulosa cell)
KW - Adrenaline
KW - Catecholamine
KW - Ornithine decarboxylase
KW - β-Adrenergic stimulation
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U2 - 10.1016/0304-4165(80)90314-1
DO - 10.1016/0304-4165(80)90314-1
M3 - Article
C2 - 6153144
AN - SCOPUS:0018907074
SN - 0304-4165
VL - 627
SP - 123
EP - 130
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 2
ER -