β-2-adrenergic stimulation of ornithine decarboxylase activity in porcine granulosa cells in vitro

Johannes D Veldhuis, Timothy S. Harrison, James M. Hammond

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Epinephrine, norepinephrine, and isoproterenol produced dose-dependent stimulation of ornithine decarboxylase (EC 4.1.1.7) activity in isolated porcine granulosa cells maintained under defined conditions in vitro. β- but not α-receptor-blocking agents prevented enzyme stimulation by catecholamines. Application of preferential β-1 and β-2-receptor antagonists and agonists localized the epinephrine effect to β-2-adrenergic mediation. Epinephrine action was enhanced by the phosphodiesterase inhibitor, 1-methyl-3-isobutyl-xanthine, but not by saturating concentrations of the cyclic AMP analogue, 8-bromocyclic AMP, of follicle-stimulating hormone, or of prostaglandin E2. However, stimulation by epinephrine was additive to that of luteinizing hormone. Follicular fluid obtained from immature Graafian follicles contined concentrations of norepinephrine and epinephrine active in vitro. Thus, catecholamines may participate in the regulation of ornithine decarboxylase activity in the ovary. Catecholamine effects may be mediated by β-2-receptors linked to the adenylate cyclase system.

Original languageEnglish (US)
Pages (from-to)123-130
Number of pages8
JournalBBA - General Subjects
Volume627
Issue number2
DOIs
StatePublished - Jan 17 1980
Externally publishedYes

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Ornithine Decarboxylase
Granulosa Cells
Adrenergic Agents
Epinephrine
Swine
Catecholamines
benzoylformate decarboxylase
Norepinephrine
Follicular Fluid
Xanthine
Ovarian Follicle
Phosphodiesterase Inhibitors
Follicle Stimulating Hormone
Adenosine Monophosphate
Luteinizing Hormone
Isoproterenol
Dinoprostone
Adenylyl Cyclases
Cyclic AMP
Ovary

Keywords

  • (Porcine granulosa cell)
  • Adrenaline
  • Catecholamine
  • Ornithine decarboxylase
  • β-Adrenergic stimulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

β-2-adrenergic stimulation of ornithine decarboxylase activity in porcine granulosa cells in vitro. / Veldhuis, Johannes D; Harrison, Timothy S.; Hammond, James M.

In: BBA - General Subjects, Vol. 627, No. 2, 17.01.1980, p. 123-130.

Research output: Contribution to journalArticle

Veldhuis, Johannes D ; Harrison, Timothy S. ; Hammond, James M. / β-2-adrenergic stimulation of ornithine decarboxylase activity in porcine granulosa cells in vitro. In: BBA - General Subjects. 1980 ; Vol. 627, No. 2. pp. 123-130.
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