βγ subunits mediate the NPY enhancement of ATP-stimulated inositol phosphate formation

Xinying Li, Tsuneya Ikezu, Terry D. Hexum

Research output: Contribution to journalArticlepeer-review

Abstract

Neuropeptide Y (NPY) enhances ATP-stimulated inositol phosphate (InsP) formation in bovine chromaffin cells through an unknown mechanism. Chromaffin cells were transduced with the carboxyl terminus of β-adrenergic receptor kinase 1 (βARK1CT), a Gβγ subunits scavenger, using a recombinant adenovirus system. The adenovirus also expresses a green fluorescent protein (GFP) which serves as an index of transduction. Flow cytometry showed that up to 80% of chromaffin cells were transduced by the virus. There was a direct correlation between the βARK1CT inhibition of the NPY enhancement of ATP-stimulated InsP formation and the percent of cells expressing GFP (r2=0.9993). These results demonstrate that Gβγ subunits are required for the NPY enhancement of ATP-stimulated InsP formation in bovine chromaffin cells.

Original languageEnglish (US)
Pages (from-to)267-274
Number of pages8
JournalPeptides
Volume25
Issue number2
DOIs
StatePublished - Feb 2004

Keywords

  • ATP
  • CAMP
  • Chromaffin cell
  • Cyclic AMP
  • DMEM
  • Dulbecco's modified Eagle medium
  • GFP
  • Green fluorescent protein
  • Inositol phosphate
  • InsP
  • MOI
  • Multiplicity of infection
  • NPY
  • NPY receptor
  • Neuropeptide Y
  • P2Y
  • Purinergic
  • Purinergic receptor
  • Y1

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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