Abstract
BALB/c mice express abnormally high levels of α-skeletal actin in the heart, which may be related to a duplication in the promoter of the α- cardiac actin gene. To evaluate the effects of overexpression of the α- skeletal actin isoform on cardiac contractile function, we studied these mice using the isolated perfused work-performing murine heart model and measured actin isoform expression in the same hearts. We quantified myocardial contractility from the maximum rate of contraction (+dP/dt) and time to peak pressure and relaxation from -dP/dt and time to half relaxation of left intraventricular pressure. Dot blots of total RNA hybridized against oligonucleotide sequences specific for either α-skeletal or α-cardiac actin mRNA showed that increased levels of α-skeletal actin RNA correlated significantly with increased contractility of hearts from the BALB/c mice (r=.80, n=15, P<.001). The present study demonstrates a significant functional correlation between α-actin isoform content and cardiac contractile function and also that α-skeletal actin may promote an increased contractile function in the heart compared with α-cardiac actin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 740-746 |
| Number of pages | 7 |
| Journal | Circulation Research |
| Volume | 74 |
| Issue number | 4 |
| State | Published - Apr 1994 |
| Externally published | Yes |
Fingerprint
Keywords
- α-actin
- mouse hearts
- myocardial function
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
α-Skeletal actin is associated with increased contractility in the mouse heart. / Hewett, Timothy; Grupp, Ingrid L.; Grupp, Gunter; Robbins, Jeffrey.
In: Circulation Research, Vol. 74, No. 4, 04.1994, p. 740-746.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - α-Skeletal actin is associated with increased contractility in the mouse heart
AU - Hewett, Timothy
AU - Grupp, Ingrid L.
AU - Grupp, Gunter
AU - Robbins, Jeffrey
PY - 1994/4
Y1 - 1994/4
N2 - BALB/c mice express abnormally high levels of α-skeletal actin in the heart, which may be related to a duplication in the promoter of the α- cardiac actin gene. To evaluate the effects of overexpression of the α- skeletal actin isoform on cardiac contractile function, we studied these mice using the isolated perfused work-performing murine heart model and measured actin isoform expression in the same hearts. We quantified myocardial contractility from the maximum rate of contraction (+dP/dt) and time to peak pressure and relaxation from -dP/dt and time to half relaxation of left intraventricular pressure. Dot blots of total RNA hybridized against oligonucleotide sequences specific for either α-skeletal or α-cardiac actin mRNA showed that increased levels of α-skeletal actin RNA correlated significantly with increased contractility of hearts from the BALB/c mice (r=.80, n=15, P<.001). The present study demonstrates a significant functional correlation between α-actin isoform content and cardiac contractile function and also that α-skeletal actin may promote an increased contractile function in the heart compared with α-cardiac actin.
AB - BALB/c mice express abnormally high levels of α-skeletal actin in the heart, which may be related to a duplication in the promoter of the α- cardiac actin gene. To evaluate the effects of overexpression of the α- skeletal actin isoform on cardiac contractile function, we studied these mice using the isolated perfused work-performing murine heart model and measured actin isoform expression in the same hearts. We quantified myocardial contractility from the maximum rate of contraction (+dP/dt) and time to peak pressure and relaxation from -dP/dt and time to half relaxation of left intraventricular pressure. Dot blots of total RNA hybridized against oligonucleotide sequences specific for either α-skeletal or α-cardiac actin mRNA showed that increased levels of α-skeletal actin RNA correlated significantly with increased contractility of hearts from the BALB/c mice (r=.80, n=15, P<.001). The present study demonstrates a significant functional correlation between α-actin isoform content and cardiac contractile function and also that α-skeletal actin may promote an increased contractile function in the heart compared with α-cardiac actin.
KW - α-actin
KW - mouse hearts
KW - myocardial function
UR - http://www.scopus.com/inward/record.url?scp=0028178197&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028178197&partnerID=8YFLogxK
M3 - Article
C2 - 8137509
AN - SCOPUS:0028178197
VL - 74
SP - 740
EP - 746
JO - Circulation Research
JF - Circulation Research
SN - 0009-7330
IS - 4
ER -