Project: Research project

Project Details


The overall objective of this research project is to determine whether
genetic alterations in pathways of sodium ion (Na) transport in the red
blood cvells (RBC) of children can predict their risk of developing primary
hypertension (PH) in adulthood. The proposed investigation focuses on one
characteristic of in vitro Na transport in the human RBC-maximal rate of
sodium-lithium counter-transport (Na-Li CNT). This trait will first be
measured in a random sample of 1,500 school children between 7 and 18 years
of age in Rochester, Minnesota, to characterize the distribution in the
population at large. The utility of increased Na-Li CNT as a predictor of
elevated blood pressure (BP) in the young will be determined and compared
with other predictive markers (e.g., age, weight, sex). Next,
approximately 300 children will be randomly selected as index cases for
familiy studies which will assess the contribution of genetic factors to
the inter-individual variability of Na-Li CNT in the population. Complex
segregation analysis of pedigree data will be carried out to determine the
most likely mode for genetic transmission of the Na-Li CNT phenotype.
Specifically, the hypothesis that there is a single gene locus segregating
for two alleles having a major effect on Na-Li CNT will be tested.
Finally, the baseline genetic information about Na-Li will be related to
the prevalence of primary hypertension in the families of index children.
The multiple logistic regression model will be used to ask whether Na-Li
CNT levels in index children and for aggregation of Na-Li CNT levels in
their first-degree relatives can predict prevalence of primary hypertension
in the adult members of the family. Information regarding Na-Li CNT and
blood pressure obtained from these studies will be applicable to the white
population of the United States in general. This research will help
elucidate the genetic basis of alterations in membrane Na transport in the
human RBC and may lead to simple methods to help identify children at
increased risk of developing primary hypertension in adulthood
Effective start/end date4/1/843/31/97


  • Medicine(all)