RNA Decoys for DNA Binding Proteins

Project: Research project

Description

There is growing appreciation that small, non-coding RNAs can participate in gene regulation. Can small RNAs inhibit DNA-binding proteins? We have developed an artificial example by performing in vitro genetic selection experiments identifying a small RNA aptamer that competitively inhibits human transcription factor NF-kappaB binding to DNA in vitro. Optimization by yeast in vivo genetic selections resulted in an RNA that inhibits NF-kappaB in living yeast cells. We have solved the X-ray co-crystal structure of this unusual RNA/NF-I
StatusFinished
Effective start/end date2/1/041/31/09

Funding

  • National Institutes of Health: $279,714.00
  • National Institutes of Health: $295,000.00
  • National Institutes of Health: $288,068.00
  • National Institutes of Health: $295,000.00

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NF-kappa B
Genetic Selection
DNA-Binding Proteins
Yeasts
Nucleotide Aptamers
RNA
Small Untranslated RNA
Transcription Factors
X-Rays
DNA
Genes
In Vitro Techniques
immune RNA

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)