PILOT--A MODEL FOR THE EXPERIMENTAL ANALYSIS OF DERMAL VASCULATURE

Project: Research project

Project Details

Description

Many of the disease processes which affect skin have a prominent vascular
component. This is especially true of acute and chronic inflammatory
diseases of both immune and nonimmune etiologies. Recent in vitro studies
have established the importance of endothelial activation in inflammatory
processes in which endothelial- leukocyte interactions are a prominent
feature. A critical event in this activation response is the rapid and
specific induction of expression of endothelial-leukocyte adhesion
molecules such as E-Selectin. In this project, we propose to evaluate the
feasibility of combining genetic manipulation of murine embryonic stem
cells with blastocyst-mediated chimera formation as a novel model for
investigating vascular biologic features of skin disease. In support of
this overall goal we will pursue the following Specific Aims:

1. Generation of E-Selectin/Chimeric mice for analysis of dermal
vasculature;

2. Determination of lymphocyte homing in models of endothelial activation;

3. Application of E-Selectin/Chimeric mice to basic studies of skin
pathophysiology.

If successful, these studies are expected to provide fundamentally new
insights into the cutaneous biology and pathophysiology of the dermal
microvasculature.
StatusNot started

Funding

  • National Institutes of Health

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