Molecular functions of Tcf-1 in DP thymocytes

PROJECT NARRATIVE Nearly every stage of T cell development critically depend on Tcf-1, a multifunctional protein, expressed in distinct isoforms, that regulates of the chromatin landscape, conformation and gene expression. The proposed studies will address the hypothesis that Tcf-1 participates in distinct regulatory protein complexes and cooperates with Lef-1 and HEB to dynamically modulate chromatin conformation and expression profiles that guide thymocyte development. Determining how these regulators co-ordinate to establish epigenetic and chromatin conformation states will provide a novel and comprehensive molecular model of how DP thymocyte programs are modulated.