Molecular Detection of Biofilms on Hip and Knee Implants

Project: Research project

Description

DESCRIPTION (provided by applicant): Approximately 750,000 total hip and knee replacement surgeries are performed each year in the United States. As a result of the aging population, these numbers are expected to increase to two and a half million by 2030. While the majority of those who undergo these procedures experience dramatic benefit, approximately three percent require re-operation, most commonly for prosthetic joint infection (PJI) or aseptic loosening of the prosthesis. Surgical and medical management of failed joint replacements depend on whether or not infection is present. However, making a microbiologic diagnosis of PJI is challenging. We have shown that native joint infection diagnostics are inaccurate for PJI. Microorganisms associated with PJI are found in biofilms on the prosthesis surface;accordingly, methods that sample the prosthesis surface should improve the diagnosis of PJI. Our research team published a manuscript in the New England Journal of Medicine, showing that a novel technique that that we have developed, which samples biofilms on the prosthesis surface, is more sensitive than conventional periprosthetic tissue for the culture-based diagnosis of PJI. Although this technique is more sensitive than conventional approaches, it is culture-based, and, accordingly has a long turnaround time. Further, despite improved sensitivity compared to periprosthetic tissue culture (i.e., 78.5 versus 60.8%, p
StatusActive
Effective start/end date12/1/083/31/20

Funding

  • National Institutes of Health: $238,500.00
  • National Institutes of Health: $269,260.00
  • National Institutes of Health: $547,302.00
  • National Institutes of Health: $271,980.00
  • National Institutes of Health: $547,302.00
  • National Institutes of Health: $245,565.00
  • National Institutes of Health: $258,490.00
  • National Institutes of Health: $258,490.00

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Biofilms
Joints
Hip
Knee
Infection
Metagenomics
Arthroplasty
Prostheses and Implants
Knee Replacement Arthroplasties
Replacement Arthroplasties
Bacteria
Equipment Failure
Prosthesis Failure
Orthopedics
High-Throughput Nucleotide Sequencing
Polymerase Chain Reaction
New England
Manuscripts
Hip Replacement Arthroplasties
Medicine

ASJC

  • Medicine(all)