MOLECULAR CYTOLOGY OF A CA-BINDING CONTRACTILE PROTEIN

Project: Research project

Description

Striated flagellar roots occur associated with the basal apparatus of
flagellated or ciliated eukaryotic cells and have been observed in
association with centrioles and primary cilia. Striated flagellar roots
are contractile organelles. We have recently demonstrated that striated
flagellar roots are composed in large part of a calcium-binding 20,000 mol.
wt. contractile phosphoprotein. This protein shares many features of the
parvalbumin/troponin c/calmodulin superfamily of calcium-binding proteins.
We present preliminary findings which suggest that antigenic homologs of
the striated flagellar root protein occur associated with the centrioles
and mitotic spindle poles of diverse organisms, including mammalian cells.
Our ultrastructural studies indicate that the fibrous material and their
condensations (pericentriolar satellites) of mammalian centrosomes is
composed at least in part of antigenic homologs of striated flagellar
roots. These findings have led to the proposal that striated flagellar
root homologs respresent 'primitive' motile systems that are simple in
composition and undergoe contraction directly in response to calcium
binding. If these ideas are correct they have important implications for
our understanding of eukaryotic cell structure and contractile function.
We propose to characterize the antigenic homologs of the striated flagellar
root protein in mammalian cells. We propose to generate a library of
monoclonal antibodies and to use these in epitope mapping,
immunofluorescent and immunoelectron microscopic studies of striated
flagellar root homologs in mammalian cells. In addition we will
characterize calcium-binding to the protein and its proteolytic fragments
and study the characteristics of flagellar root protein phosphorylation.
StatusFinished
Effective start/end date8/30/857/31/93

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Contractile Proteins
Cell Biology
Carrier Proteins
Calcium-Binding Proteins
Eukaryotic Cells
Proteins
Spindle Poles
Centrioles
Epitope Mapping
Centrosome
Spindle Apparatus
Troponin
Cilia
Phosphoproteins
Calmodulin
Organelles
Libraries
Phosphorylation
Calcium
Antibodies

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)