HORMONE ACTION IN THE OVARY: ROLE OF CALCIUM IONS

  • Veldhuis, Johannes D (PI)

Project: Research project

Project Details

Description

Our commitment to studying the role of calcium effector pathways in ovarian
differentiation will be continued by focusing on the regulatory actions of
the calcium-phospholipid-diacylglycerol (PKC) second-messenger system in a
well defined and established in vitro model of swine granulosa cells that
retain responsivity to a variety of hormonal agonists. We will
particularly investigate the manner in which FSH and angiotensin II
(presumptively acting respectively through distinct cyclic AMP and PKC
intracellular signaling pathways) alter intracellular calcium ion
concentrations in single porcine granulosa cells (Time-resolved
fluorescence video-microscopy); how hormonal agonists trigger increased
secretory activity by single ovarian cells (reverse hemolytic plaque assay
of relaxin secretion by pig luteal cells); employ a microperifusion system
to test the temporal concordance between intracellular calcium-ion
concentration changes and secretory events; probe the causal association
between increased intracellular calcium-ion concentrations and resultant
secretion using experimentally induced calcium transients in permeabilized
cells; and begin to dissect the important interactions between the calcium
effector signaling pathway and the cyclic AMP second messenger pathway on
sterol-metabolizing enzymes. These emphases reflects work carried out
under my parallel RCDA, in which I have has occasion to learn appropriate
single-cell and reproductive biology techniques that can now be applied to
this R01 renewal. The significance of the calcium second-messenger system
in the ovary will therefore be explored at the single cell level (Specific
Aim I), in relation to calcium's causal relevance to hormone secretion
(Specific Aim II), and in relation to the interactions between the PKC and
classical cyclic AMP effector pathways (interactions to be studied at the
level of steroidogenesis). Overall, we anticipate that these approaches
will yield significant and interesting new information regarding ovarian
cellular physiology, which will enhance our physiological understanding or
reproductive biology and fertility regulation in higher mammals.
StatusFinished
Effective start/end date4/1/844/30/01

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $182,934.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $52,024.00

ASJC

  • Medicine(all)

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