EXPERIMENTAL CHRONIC ENTRAPMENT NEUROPATHY

Project: Research project

Project Details

Description

The entrapment neuropathies are the most common type of neuropathies
affecting man and cause major personal and economic impact. We have
developed a model of chronic entrapment in rats and rabbits which regularly
cause focal demyelination. Methodology has been developed in this
laboratory to directly measure endoneurial fluid pressure and blood flow
within the entrapped region. By relating endoneurial fluid pressure and
blood flow values to the focal pathologic changes, it will be possible to
directly test the hypothesis that entrapment induced demyelination is
caused by local and focal collapse of capillaries or other ischmic
mechanisms. By systematically relating pressure: volume changes to axon
volume and shape changes at multiple levels using detailed morphometry it
will be possible to define the type an quantity of biophysical stresses
that cause demyelination. This approach has previously not been possible.
Peripheral nerve perineurium is distorted at the cuff edge and should
perineurial permeability or compliance become abnormal at these regions
there may occur an influx of potentially neurotoxic compounds into the
endoneurium of nerve. We plan to directly measure perineurial compliance
of the entrapped region using a sensitive in vitro active servo-null system
and to test if permeability is increased using two isotopes (C14 sucrose,
I125 albumin) in entrapped and normal fibers and the same fibers when
stressed to 5 mm mercury. The endoneurial fluid pressure and morphometric
studies will be performed in year 1, compliance and permeability studies
in year 2 and intrafascicular blood flow studies in year 3.
StatusFinished
Effective start/end date4/1/833/31/86

Funding

  • National Institutes of Health

ASJC

  • Medicine(all)
  • Neuroscience(all)

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