EOSINOPHIL EFFECTOR FUNCTIONS IN THE LUNG

Project: Research project

Project Details

Description

The focus of the proposal is to understand the relationship between
allergic pulmonary inflammation and the recruitment/activation of
eosinophils in the airway mucosa and lumen. Our objectives are to
identify those lung pathologies attributable to the eosinophil and then
focus on the effector functions mediated by individual eosinophil
secondary granule proteins. The specific aims of this proposal are: (1)
To determine if the recruitment of eosinophils to the airway mucosa and
lumen of antigen-challenged mice leads to pulmonary pathologies. This
aim will be accomplished using a two-fold strategy- (i) The assessment
of pathologies occurring in ovalbumin (OVA) sensitized/challenged IL-5
knock-out mice with and without adoptive engraftment of eosinophils
(i.e., the correlation of specific pathologies to the presence of
eosinophils) and (ii) The determination of pulmonary changes mediated
by eosinophils in an OVA sensitized/challenged model using mice with a
unique transgene-mediated ablation of the eosinophil lineage (i.e., the
lack of specific pathologies in the absence of eosinophils); (2) To
assess the pulmonary effects of introducing murine eosinophil secondary
granule proteins into the lung; (3) To characterize the effects of an
eosinophil major basic protein (mMBP) gene knock-out in mouse models of
OVA induced pulmonary inflammation. The animal models characterized in
this proposal were developed to extend clinical studies of asthmatic
patients. These models provide unique opportunities to test hypotheses
of eosinophil effector functions occurring in pulmonary allergic
inflammation.
StatusFinished
Effective start/end date7/15/986/30/02

ASJC

  • Medicine(all)