EOSINOPHIL ACTIVITIES IN MURINE MODELS OF LUNG DISEASE

Project: Research project

Project Details

Description

DESCRIPTION (Adapted from the applicant's abstract)

The aims of this proposal are to provide the research community with high
throughput phenotypic assays in the mouse to detect the presence of
eosinophils and/or eosinophil-mediated activities. These objectives utilize
unique transgenic and gene knockout animals to develop eosinophil-specific
reagents, creating three independent strategies for screening large numbers of
mice in studies of allergic inflammation such as pulmonary models of asthma:
(I) rabbit polyclonal antisera for histological screens of paraffin sections;
(ii) rat/mouse monoclonal antibodies for sensitive sandwich ELISA assays to
quantify eosinophil activities (e.g., eosinophil degranulation in BAL
samples); (iii) generation/identification of monoclonal antibodies against
cell surface epitopes specific for "activated" eosinophils in FACS analyses of
eosinophil tissue/organ recruitment (e.g., recruitment of activated
eosinophils to the airway lumen in mouse models of pulmonary inflammation).
In the short-term, these strategies represent independent endpoint
assessments. However, in the context of a larger scientific community
analyzing different murine model systems, the results from these strategies
will generate benchmark parameters for high throughput evaluations of
inflammatory responses. Thus, the reagents/methodologies developed in this
proposal uniquely provide a multifaceted approach investigators will use in
rapid screens of large numbers of animals. Moreover, by correlating these
reproducible and quantitative assays of defined inflammatory markers with more
complex phenotypic assessments, investigators will create indices with which
detailed evaluation of mouse models are achieved in a quick cost-effective
fashion. (End of Abstract.)
StatusFinished
Effective start/end date9/30/007/31/18

Funding

  • National Heart, Lung, and Blood Institute: $415,000.00

ASJC

  • Medicine(all)

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