Project: Research project

Project Details


DESCRIPTION Among the more remarkable
features of the MHC is the extensive structural diversity present both in
the organization of gene clusters within the class I and class II multigene
families and in molecules. Many lines of evidence indicate that structural
diversity is reflected in functional diversity among the MHC glycoproteins.
Understanding MHC diversity is one key to deciphering how the immune system
functions. The long-term goal of the presented studies is to determine the
structure and origin of MHC diversity and to relate this diversity to
immune function. The specific aims of this proposal are: 1) to determine
whether there exists conserved genes with potential functional significance
within the T1 region of the murine MHC; 2) to characterize the extent of
the deletion within the Q region of the f-haplotype and establish its
frequency in wild populations; 3) to determine the nucleotide sequence for
all the uncharacterized K and D loci among the commonly studied inbred
lines as well as for a select group of members of different wild mouse
species; 4) to determine whether a previously identified copy mechanism
that generates sequence diversity at the K and D loci introduces random
mutations during the copy event; 5) to develop a molecular
genetically-based approach to the analysis of the occurrence of spontaneous
MHC mutants within mouse lines; and 6) to establish a cell culture system
that permits the investigation of the genetic mechanisms driving gene
conversion-like events among MHC genes.
Effective start/end date7/1/858/31/97


  • Medicine(all)
  • Immunology and Microbiology(all)