CYTOKINE INDUCED SIGNALING AND CELL PROLIFERATION

Project: Research project

Description

DESCRIPTION The Specific Aims of the proposal are: 1) To dissect the role of PI3-K in the regulation of signalling protein (MEK and MAPK) activation in hematopoietic cells using constitutively active and dominant negative PI3-K mutants. 2) To biochemically and genetically identify the novel MEK activator in the wortmannin-sensitive, IL-3-inducible MEK activation pathway. 3) To determine the roles of MEK and MAPK and upstream regulators in hematopoietic cell proliferation and survival using transient and long-term proliferation assays. These studies are designed to characterize a discreet wortmannin-sensitive MAPK activation pathway that based on preliminary data may have cell type-specific components. Once identified these components will provide novel signal-transducing targets for pharmacological intervention to block cytokine-dependent proliferation of leukemic and normal myeloid lineage cells.
StatusFinished
Effective start/end date4/5/973/31/03

Funding

  • National Institutes of Health: $103,268.00
  • National Institutes of Health
  • National Institutes of Health: $93,971.00
  • National Institutes of Health: $106,614.00
  • National Institutes of Health: $100,048.00

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Mitogen-Activated Protein Kinase Kinases
Cell Proliferation
Cytokines
Interleukin-3
Myeloid Cells
Cellular Structures
Cell Survival
Pharmacology
Proteins
wortmannin

ASJC

  • Medicine(all)