CELLULAR BIOLOGY OF THE TRABECULAR MESHWORK

Project: Research project

Description

A new method for in situ organ culture of human trabecular
meshwork will be used to study the cellular biology of the
trabecular meshwork. The new technique allows the anterior
segment of eyes to maintain a relatively constant intraocular
pressure while being perfused with culture media. The media flow
through the trabecular meshwork in a normal manner, enter
Schlemm's canal, and finally leave the eye through the collector
channels. Trabecular meshwork structure and cellularity are
maintained for at least four weeks in culture as determined by
light and electron microscopy. Cells remain metabolically active,
incorporating the amino acid 3H leucine and the proteoglycan
precursor 3H glucosamine after four weeks of culture. This
technique enables controlled experiments to be performed on
intact human trabecular meshwork perfused in a normal fashion.
The proposed grant will allow refinement of the present culture
technique, development of parameters to assess experimental
changes, and study of several basic questions about human
trabecular meshwork. The effect of corticosteroid, epinephrine, and pilocarpine on
human trabecular meshwork will be studied with this culture
technique. Normal human eyes obtained at autopsy will be used,
as well as human glaucomatous eyes. Changes induced by these
agents will be studied using one eye as an untreated control, the
fellow eye as the treated experimental eye. In addition,
trabecular biopsies obtained via surgical trabeculectomy will be
used to monitor changes during the course of the experiment. Structural appearance will be studied with both light and electron
microscopy (transmission and scanning). Changes in protein and
extracellular matrix synthesis will be monitored
autoradiographically using radioisotope tracers and biochemically
using analytical techniques. The effect of intraocular pressure, ascorbate, and growth factor,
will be studied in separate experiments. Pairs of eyes will be
cultured with one eye receiving the experimental agent, and the
fellow cultured eye serving as the untreated control eye. The
trabecular response to commonly found intraocular debris (blood,
pigment, lens cortex) will be studied in a similar fashion, making
special note of cell loss and replication.
StatusActive
Effective start/end date7/1/87 → …

Funding

  • National Institutes of Health: $576,410.00
  • National Institutes of Health: $597,670.00
  • National Institutes of Health: $343,063.00
  • National Institutes of Health: $602,693.00
  • National Institutes of Health: $575,144.00

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Trabecular Meshwork
Cell Biology
Extracellular Matrix
Prostaglandins
Microscopy
Transforming Growth Factor beta2
Organized Financing
Glaucoma

ASJC

  • Medicine(all)