CCK QUANTIFICATION AND RECEPTOR CHARACTERIZATION

Project: Research project

Description

The long-range goal of this proposal is to understand the role
cholecystokinin (CCK) plays in health and disease. This specific proposal
concentrates on the levels of the CCK peptides, their receptors in various
tissues, and peptide-receptor interactions. There are two specific and
inter-related aims: 1) development of methodology to quantify CCK and its
molecular species, and 2) development of methodology to characterize CCK
receptors in various tissues both functionally and biochemically.
Quantification of CCK will be attempted using a) radioimmunoassay with
conventional polyclonal antisera, b) radioimmunoassay with monoclonal
antisera, and c) radioreceptor assay. Molecular species of CCK will be
separated by high performance liquid chromatography. CCK receptors will be
characterized functionally using ligand binding techniques, and
biochemically using affinity labeling and chemical crosslinking. These
approaches will add basic information about this physiologically important
gastrointestinal hormone, and provide means to directly evaluate the role
this hormone plays in health and disease. Screening of CCK and its
receptor function may permit identification of abnormalities which mediate
pathologic processes in man.
StatusFinished
Effective start/end date8/1/837/31/86

Funding

  • National Institutes of Health

Fingerprint

Cholecystokinin Receptors
Peptide Receptors
Radioimmunoassay
Hormones
Radioligand Assay
Health
Immune Sera
High Pressure Liquid Chromatography
Ligands

ASJC

  • Medicine(all)