A NEW CLASS OF EPSTEIN BARR VIRUS NUCLEAR ANTIGEN

  • Spelsberg, Thomas C, (PI)

Project: Research project

Description

A new class of Epstein Barr Virus Nuclear Antigen (Class II) has been found
which is more tightly bound to chromatin than the standard EBNA (Class I). This
Class II EBNA has different antigenic and physicochemical properties than the
Class I EBNA. Also, standard histones do not appear to react with these
antisera. The Class II EBNA appears to be a modified histone or histone-like
protein. It is planned to purify the Class II EBNA by the techniques described
in the progress report, e.g., isoelectric focusing, molecular sieve and ion
exchange chromatography, and preparative polyacrylamide gel electrophoresis. It
is hoped to determine whether this antigen(s) is histone(s), whether it is
modified by phosphorylation, acetylation, etc., or whether it is a histone-like
protein. In the future it is planned to prepare antisera against this Class II
EBNA.
StatusFinished
Effective start/end date4/1/7912/31/85

Funding

  • National Institutes of Health

Fingerprint

Epstein-Barr Virus Nuclear Antigens
Histones
Isoelectric Focusing
Acetylation
Chromatin
Gel Chromatography
Immune Sera
Polyacrylamide Gel Electrophoresis
Phosphorylation
Antigens

ASJC

  • Medicine(all)