3-D ELECTRON MICROSCOPY OF MACROMOLECULES

  • Barry, Michael A (PI)
  • Esser, A. (PI)
  • Phillips, G. (PI)
  • McDowall, A. (PI)
  • Stubbs, G. (PI)
  • Dorset, D. (PI)
  • Deatherage, J. (PI)
  • Robinson, J. (PI)
  • Goldstein, M. (PI)
  • Heath, J. (PI)
  • Hawkins, H. (PI)
  • Matsudaira, P. (PI)
  • Rixon, F. (PI)
  • Davidson, A.M.Y. (PI)
  • Schmid, Michael F. (PI)
  • Zhou, Z. Hong (PI)
  • King, Stephen M. (PI)
  • Prevelige, Peter E. (PI)
  • Penczek, Pawel A. (PI)
  • Jiang, W.E.N. (PI)
  • Prasad, Bidadi V. Venkataram (PI)
  • Tsai, Francis T.F. (PI)
  • Sprang, Stephen R. (PI)
  • Zhou, Z. Hong (PI)
  • Schmid, Michael F. (PI)
  • Pownall, Henry J. (PI)
  • Ju, T.A.O. (PI)
  • Yan, H.A.O. (PI)
  • Crowther, R. (PI)
  • Hollinger, F. Blaine (PI)
  • Davidson, Amy L. (PI)
  • Stanciu, L.I.A. (PI)
  • Kavraki, Lydia E. (PI)
  • Kelly, Jeffrey A. (PI)
  • Tsai, Francis T.F. (PI)
  • Gogol, Edward P. (PI)
  • Hill, R. Blake (PI)
  • Demartino, George N. (PI)
  • Egelman, Edward H. (PI)
  • Danesh, Farhad R. (PI)
  • [No Value], X. Nancy (PI)
  • Wu, J.I.A. (PI)
  • Epstein, Henry F. (PI)
  • Epstein, Henry F. (PI)
  • Smith, Louis (PI)
  • Gilbert, Hirim (PI)
  • Michael, Way (PI)
  • Brink, Jacob (PI)
  • Buckley, Betty (PI)
  • Brinton, Margo (PI)
  • Hamilton, Susan (PI)
  • Wensel, Theodore (PI)
  • Bryan, Joseph (PI)
  • Epstein, Henry F. (PI)
  • Schmid, Michael (PI)
  • Estes, Mary Kolb (PI)
  • King, Jonathan (PI)
  • Zhou, Zheng (PI)
  • Paredes, Angel (PI)
  • Brown, Dennis (PI)
  • Dougherty, Matthew (PI)
  • Polly, Roy (PI)
  • Chiu, W.A.H. (PI)
  • Danesh, Farhad R. (PI)
  • Estes, Mary Kolb (PI)
  • Dougherty, Matthew (PI)
  • Chiu, W.A.H. (PI)
  • Esser, A. (PI)
  • Phillips, G. (PI)
  • McDowall, A. (PI)
  • Stubbs, G. (PI)
  • Dorset, D. (PI)
  • Smith, Louis (PI)
  • Deatherage, J. (PI)
  • Robinson, J. (PI)
  • Goldstein, M. (PI)
  • Heath, J. (PI)
  • Hawkins, H. (PI)
  • Gilbert, Hirim (PI)
  • Brink, Jacob (PI)
  • Brinton, Margo (PI)
  • Hamilton, Susan (PI)
  • Wensel, Theodore (PI)
  • Epstein, Henry F. (PI)
  • Schmid, Michael (PI)
  • Zhou, Zheng (PI)
  • Paredes, Angel (PI)
  • Polly, Roy (PI)
  • Matsudaira, P. (PI)
  • Rixon, F. (PI)
  • Aebi, Ueli (PI)
  • Sherman, Michael (PI)
  • Lu, Guang (PI)
  • Smith, Lou (PI)
  • Black, Shaun (PI)
  • McGough, Amy (PI)
  • Pedersen, Peter (PI)
  • Davidson, A.M.Y. (PI)
  • Ludtke, Steven (PI)
  • Brink, Jacob (PI)
  • Baldwin, Phillip (PI)
  • Wakil, Salih (PI)
  • Hamilton, Susan (PI)
  • Wensel, Theodore (PI)
  • Sherman, Michael (PI)
  • Epstein, Henry F. (PI)
  • McGough, Amy (PI)
  • Pedersen, Peter (PI)
  • Schmid, Michael F. (PI)
  • Black, Shaun (PI)
  • Estes, Mary Kolb (PI)
  • Zhou, Z. Hong (PI)
  • Paredes, Angel (PI)
  • Lu, Guang (PI)
  • Brown, Dennis (PI)
  • Smith, Lou (PI)
  • Dougherty, Matthew (PI)
  • Khant, Htet (PI)
  • King, Stephen M. (PI)
  • Prevelige, Peter E. (PI)
  • Booth, Christopher (PI)
  • Holzenburg, Andreas (PI)
  • Brown, Dennis (PI)
  • Y Jane, Tao (PI)
  • Glaeser, Robert (PI)
  • Epstein, Henry F. (PI)
  • Pedersen, Peter (PI)
  • Gutsche, Irina (PI)
  • Quiocho, Florante (PI)
  • Witman, George (PI)
  • Akey, Christopher (PI)
  • Penczek, Pawel A. (PI)
  • Jiang, W.E.N. (PI)
  • Hamilton, Susan (PI)
  • Rizo, Jose (PI)
  • Prasad, Bidadi V. Venkataram (PI)
  • Watowich, Stanley (PI)
  • Wakil, Salih Jawad (PI)
  • Tsai, Francis T.F. (PI)
  • Sprang, Stephen R. (PI)
  • Dougherty, Matthew (PI)
  • Barford, David (PI)
  • Zhou, Z. Hong (PI)
  • Chuang, David (PI)
  • Jacobson, Raymond (PI)
  • Ka Yee Yeechristina, Lee (PI)
  • Wensel, Theodore (PI)
  • Frydman, Judith (PI)
  • Ludtke, Steven (PI)
  • Schmid, Michael F. (PI)
  • Serysheva, Irina (PI)
  • Serwer, Philip (PI)
  • Pownall, Henry J. (PI)
  • Ju, T.A.O. (PI)
  • Kennedy, Keiron (PI)
  • Xiangwei, He (PI)
  • Berman, Helen (PI)
  • Bajaj, Chandrajit (PI)
  • Sali, Andrej (PI)
  • Halas, Naomi (PI)
  • Pati, Debananda (PI)
  • Yan, H.A.O. (PI)
  • Zechiedrich, Elizabeth Lynn (PI)
  • Lopez, Jose Aron (PI)
  • King, Jonathan Alan (PI)
  • Crowther, R. (PI)
  • Wilson, Lon (PI)
  • Sosinsky, Gina (PI)
  • Baker, David (PI)
  • Levy, Gary (PI)
  • Wittung-Stafshede, Pernilla (PI)
  • Baker, Marc (PI)
  • Holzenburg, Andreas (PI)
  • Rout, Michael (PI)
  • Holmes, Kathryn (PI)
  • Hollinger, F. Blaine (PI)
  • Davidson, Amy L. (PI)
  • Stroud, Robert (PI)
  • Shively, Jessup (PI)
  • McKnight, Steven (PI)
  • Trent, Jonathan (PI)
  • Frieden, Carl (PI)
  • Hays, Rye (PI)
  • Decker, Heinz (PI)
  • Sosnick, Tobin (PI)
  • Chisholm, Sally (PI)
  • Fletcher, Daniel (PI)
  • Wigley, Dale (PI)
  • Roberson, Robert (PI)
  • Ferrari, Mauro (PI)
  • Kao, Cheng (PI)
  • Stanciu, L.I.A. (PI)
  • Wilson, Graeme (PI)
  • Chen, Meei-Shia (PI)
  • Raney, Kevin (PI)
  • Gamblin, Truman (PI)
  • Glaeser, Robert (PI)
  • Yang, Chin- (PI)
  • McCarley, Robin Lindsey (PI)
  • Summers, Michael Finley (PI)
  • Sheetz, Michael Patrick (PI)
  • Stoilova-McPhie, Svetla (PI)
  • Kavraki, Lydia E. (PI)
  • Acehan, Devrim (PI)
  • Andersen, Felicie (PI)
  • Akey, Christopher (PI)
  • Kelly, Jeffrey A. (PI)
  • Kloc, Malgosia (PI)
  • Wittung-Stafshede, Pernilla (PI)
  • Lee, Wen- (PI)
  • Yizhi Jane, Tao (PI)
  • Sali, Andrej (PI)
  • Zasadzinski, Joseph Anthony (PI)
  • Hamilton, Susan (PI)
  • Cynthia, He (PI)
  • Prasad, Bidadi Venkataram (PI)
  • Baker, David (PI)
  • Lloyd, Richard (PI)
  • Berman, Helen (PI)
  • Baker, Marc (PI)
  • Wakil, Salih Jawad (PI)
  • Tsai, Francis T.F. (PI)
  • Weaver, Scott (PI)
  • Dougherty, Matthew (PI)
  • Safinya, Cyrus (PI)
  • Brinker, Jeffrey Alan (PI)
  • Rye, Hays (PI)
  • Tahirov, Tahir (PI)
  • Shieh, Dar- (PI)
  • Liu, Jun (PI)
  • Songi, Han (PI)
  • Tari, Ana (PI)
  • Lieber, Charles (PI)
  • Dong, Jing- (PI)
  • Gogol, Edward P. (PI)
  • Craigen, William James (PI)
  • Chen, Rong (PI)
  • Hill, R. Blake (PI)
  • Shih, Yu-Ling (PI)
  • Weber, Patricia Carol (PI)
  • Chen, Jeannie (PI)
  • Demartino, George N. (PI)
  • Mobley, William (PI)
  • Jiang, Qiu-Xing (PI)
  • Sood, Anil (PI)
  • Hayer-Hartl, Manajit (PI)
  • Kao, Cheng (PI)
  • Etkin, Laurence (PI)
  • Egelman, Edward H. (PI)
  • Sosinsky, Gina (PI)
  • Sherman, Michael (PI)
  • Bamford, Dennis (PI)
  • Oganesyan, Vaheh (PI)
  • Irvine, Darrell (PI)
  • Tirrell, Matthew (PI)
  • Yuan, Adam (PI)
  • Joachimiak, Andrzej Joachimiak (PI)
  • Falvo, Michael Richard (PI)
  • Sadler, Evan (PI)
  • Pun, Suzie (PI)
  • King, Li (PI)
  • McPherson, David (PI)
  • Eissa, Tony (PI)
  • Akey, Christopher (PI)
  • [No Value], X. Nancy (PI)
  • Wu, J.I.A. (PI)
  • Jovin, Thomas (PI)
  • Biswal, Sibani Lisa (PI)
  • McNew, James (PI)
  • King, Jonathan (PI)
  • Bryan, Joseph (PI)
  • Zhang, Qing (PI)
  • Isaya, Grazia, (PI)
  • Pownall, Henry J. (PI)

Project: Research project

Description

Our objectives are to determine the three-dimensional (3D) crystal
structures of macromolecules at near atomic resolution and the 3-D
structures of non-crystalline macromolecular assemblies at intermediate
resolution, by a combination of electron microscopy and computer processing
methods. The present proposal is a resource related project. Requests
include the acquisition of a medium high voltage (300 kV) electron
microscope with appropriate accessories and an operational fund to develop
the technology for the above aims. Technical development will be focused
on specimen preparation, 3-D data collection at low dose and low
temperature conditions and implementation of computer programs for 3-D
reconstruction. The developed technology will be immediately applied to
the high resolution crystal structure determination of crotoxin complex, T4
DNA helix destabilizing protein, fungus lysozyme and Fc fragments; and to
the intermediate resolution structure determination of muscle Z disc and
flagella axoneme. Collaborative projects will be established with
investigators outside our research groups after the midpoint of the grant
period. We plan to complete a thorough analysis of the potential as well
as limitations of this technology in its general applicability to a variety
of biological systems. We expect to present a final recommendation on the
feasibility and desirability of establishing a national shared facility of
this technology at the end of this grant period.
StatusFinished
Effective start/end date3/1/8512/31/14

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

fungi
accessories
lysozyme
muscles
cells
macromolecules
recommendations
helices
assemblies
proposals
high voltages
acquisition
resources
electron microscopy
fragments
proteins
computer programs
Sulfur
dosage
preparation

ASJC

  • Medicine(all)